Overexpression of Fyn Induces Formation of filopodia and
lamellipodia in CHO Cells via Rearrangement of F-Actin
Lei An,
Yingxue Huang, Lingzhen Song, Wei Zhang, Yamei Zhang, Shulin Chen
and Shanting Zhao*
College of Veterinary Medicine,
Northwest A&F University,
Yangling 712100,
Shaanxi, PR China *Corresponding author: shantingzhao@hotmail.com
Abstract
As a main ancillary molecules
involved in the regulation ofproliferation, differentiation and migration of
cortical neurons, Fyn has also been identified as a signal factor in motility
and growth involved with cytoskeleton. However, the molecular mechanism of Fyn on cytoskeleton remains
unclear. The results showed that the morphology of the CHO cells transfected with the recombination vector changed
remarkably. The numbers of stress fibers may be crunched to
transformation for generating and supporting the formation of filopodia and
lamellipodia, but the changes of vinculin and tubulin are unremarkable. In
addition, high concentrations of Fyn cause formation of lamellipodia as well as
filopodia. From this research we can draw a conclusion thatoverexpression of mouse Fyn induces the F-actin cytoskeleton rearrangement and provides a performance that both vinculin and tubulin are absent in the
process of Fyn-mediated F-actin reorganization.