Characterization of Two Lactic Acid Bacteria and
Their Influence on Silage Fermentation of Napiergrass
Gang Guo, Xiao-hui Sun, Xiao-yan Qiu, Masataka
Shimojo1 and Tao Shao*
Institute of Ensiling and Processing of Grass,
College of Animal Sciences and Technology, Nanjing Agricultural
University, Weigang 1, Nanjing, 210095, China; 1Laboratory
of Animal Feed Science, Department of Animal and Marine Bioresource
Sciences, Faculty of Agriculture, Kyushu University, Fukuoka
812-8581, Japan
Abstract
Enterococcus faecium
R5-1 (EF) and Lactobacillus plantarum
N30-6 (LP) isolated from silages were identified and their influence on silage
fermentation of napiergrass (Pennisetum
purpureum Sch.) harvested at various times of sunny day were studied. Strain
LP had stronger growth ability, acid tolerating capacity and wider fermentable
carbohydrates than strain EF. Napiergrass were cut at 0800, 1300 and 1800 h on a
sunny day and were inoculated with strains EF and LP at 5 log cfu g-1.
The concentrations of dry matter, water soluble carbohydrates and the ratio of
lactic acid to acetic acid (LA/AA) were higher (P<0.05) and ammonia-N (NH3-N),
acetic acid concentrations and silage pH were lower (P<0.05) for uninoculated
silages made of napiergrass cut at 1300 and 1800 h compared with cut at 0800 h.
Silages inoculated with LP and EF had lower (P<0.05) NH3-N and acetic
acid concentrations and higher (P<0.05) LA/AA than uninoculated silage made with
napiergrass cut at 1300 and 1800 h. The EF-inoculated could not improve
fermentation quality of silage made with napiergrass cut at 0800 h, which had
higher (P<0.05) NH3-N and acetic acid concentrations than
uninoculated silage. In conclusion, delayed cutting napiergrass at end of a
sunny day was associated with better fermentation quality, and the silages
inoculated with EF or LP could further improve fermentation quality.