Cutaneous epitheliotropic T-Cell Lymphoma with Multiple
Metastases in a Dog Diagnosed by PCR to Antigen Receptor
Rearrangement Analysis
Ji-SeonYoon1,§, Do-Hyeon Yu2,§,
Ru-Hui Song3,Dae Young Kim4
and Jinho Park3*
1Laboratory of
Cutaneous Aging and Hair Research, Biomedical Research Institute,
Seoul National University Hospital, 101 Daehangno, Jongno-gu,
Seoul, Korea; 2Department of Veterinary Laboratory
Medicine, College of Veterinary Medicine, Chonnam National
University,77 Yongbong-ro, Buk-gu, Gwangju, Korea; 3Department
of Veterinary Internal Medicine, College of Veterinary Medicine,
Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Korea;
4Department
of Veterinary Pathobiology, University of Missouri, 1600 E. Rollins
St, Columbia, Missouri, USA.
*Corresponding author:jpark@jbnu.ac.kr
Abstract
Herein, a case of canine cutaneous epitheliotropic T
cell lymphoma (CETL) with multiple organ metastases is described. An 11-year old female Yorkshire terrier presented with
chronic generalized dermatitis and respiratory distress. Multiple erosive
plaques and nodules in the skin, enlarged submandibular lymph nodes and
tachypnea were noted at the initial presentation. Antigen receptor rearrangement
(PARR) analysis by PCR revealed clonal rearrangements from the T-cell receptor gamma (TCRγ) in cells that
were fine-needle aspirated from the skin and submandibular lymph node.
Histopathological findings in the skin also revealed infiltration of neoplastic
lymphoid cells with epithelial tropism. Furthermore, clonal rearrangement from
the TCRγ was detected by PARR in the lung nodules. Based upon those findings,
the present case was considered as CETL with multiple
meta- stases, which has been only rarely reported in canine cases. In this case,
diagnosis of CETL and metastases to other organs could be made by PARR analysis
alone.