Comparison of Cryopreservative Effect of Different Levels of Omega-3
Egg-Yolk in Citrate Extender on the Quality of Goat Spermatozoa
N Yimer1*,
AH Noraisyah1, Y Rosnina1, H Wahid1,
K Sarsaifi1 and AM Hafizal2
1Department
of Veterinary Clinical Studies, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400, Serdang, Selangor,Malaysia; 2 Institute of National Veterinary
Biodiversity (IBVK), Jerentut, Pahang, Malaysia *Corresponding author: nurhusien@upm.edu.my
Abstract
The objective of the present study was to compare
quality of chilled and frozen-thawed goat semen processed in citrate extender
containing 3 different levels (2.5, 5 and 10%) of omega-3 egg-yolk (EY).Ejaculates were collected from five adult goats using artificial vagina.
Quality of fresh semen, processed semen after 3 hrs of chilling and 24 hrs of
freezing was assessed based on live sperm %age, abnormality (determined by
eosin-nigrosin stain) and general and progressive motility (evaluated by CEROS
computer assisted semen analyzer). The result showed a significant (P<0.05)
decrease in post-chilled live sperm %age from the fresh sample for extenders
using 2.5 and 5% EY but not for the 10%. Moreover, a significantly lower
percentage general and progressive motility was recorded using the 2.5% EY
compared to the others that showed post-chilled sperm motility non-significantly
different from the fresh sample. After chilling, 5% EY showed significantly
lower percent sperm abnormalities compared to others. However, the abnormalities
increased after freezing to a level non-significantly different from the 10% EY
that sustained to demonstrate higher live sperm %age and motility than both 2.5
and 5% EY. An overall increase in post-thawed live sperm %age, general and
progressive motility was observed with increase in concentration of EY added.
Thus, though the difference with the 5% EY is in magnitude, the 10% omega-3 EY
in citrate extender is preferred compared to 2.5% for superior post-thawed goat
semen quality, extended without washing seminal plasma.