The molecular mechanisms specifying anti-oxidative and anti-aging action of kinetin (Kn) on the splenic oxidative damage of aging rats are poorly understood. Here, the Sprague Dawley (SD) rats (Rattus norregicus) aging model was proposed by napes subcutaneous injection of D-galactose, and anti-aging group were subsequently dealt with doses of 5, 10, 20 mg/kg·BW of Kn. Superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-PX), catalase (CAT), lipid peroxide (LPO), spleen indexes and histopathology were evaluated. Our results showed that Kinetin significantly decreased the MDA and LPO activity, while spleen indexes, SOD, GSH-PX and CAT were higher than aging model group; histopathological studies for spleen confirmed the rescue from the aging rats. These results demonstrate that kinetin recovers the splenic cell structure and could effectively protect the spleen cells from oxidative damage and aging.

Key words: Aging, D-galactose, Kinetin, Oxidative damage