Close Relationship of Group A Rotaviruses between Bovine and Human Based onVP7 Gene Sequence in
Egypt
Abdallah
Merwad1, FatmaAbdallah*2 and Taisir Saber3
1Department of Zoonoses; 2Department of
Virology; 3Department
of Microbiology and Immunology, Faculty of Medicine, Zagazig
University, Egypt;
*Corresponding author:mm.fatma@yahoo.com
Abstract
The study investigated the
genetic relatedness between bovine and human group A rotaviruses. Ninety four
human stool and calf fecal samples (47, each) were collected from different
localities in Egypt
and tested by rotavirus group A enzyme immunoassay. Only twenty five fecal
samples (26.5%) were positive. Viral isolation and RT-PCR amplification of full
length VP7 gene was performed for the reacted fecal samples. VP7 genes were
sequenced for one isolate each from diarrheic human and calf. Human EGY2012 and
bovine EGY2022 rotavirus strains displayed the strongest identity of their VP7
genes [95.3% nucleotide (nt), 97.6% amino acid (aa)]. The antigenic regions A,
B, and C of its VP7 genes were analogue. The high homology of two studied
strains explained the interspecies transmission and its zoonotic hazard. Further
rotavirus P and G genotyping of human and different animal species are needed to
study genetic diversity of human rotaviruses in Egypt.
Key words:
Egypt,
Enzyme immunoassay,
Phylogenetic analysis,
Rotavirus group A, VP7 Gene