Oxidative Status and Some Serum Macro Minerals
during Estrus, Anestrous and Repeat Breeding in Cholistani Cattle
Farah Ali*, Laeeq Akbar Lodhi1, Riaz
Hussain** and Muhammad Sufyan
University College of
Veterinary and Animal Sciences, The Islamia University of
Bhawalpur-63000, Pakistan; 1Department of Theriogenology,
University of Agriculture,
Faisalabad-38040, Pakistan Corresponding authors:
*Drfarahiub@gmail.com; **driazhussain@yahoo.com
Abstract
The present study was conducted to determine the
macro mineral profile and biomarkers of oxidative stress in Cholistani cattle kept at a public farm and various
villages in district Bahawalpur. For this purpose 90 blood
samples were collected each from estrual,
anestrous and repeat breeding cattle having
different age and lactation number.
Reproductive tract examination of all the cattle was carried out to determine
the reproductive status. Blood samples without EDTA were collected for serum
separation at day of estrus (normal cyclic), repeat breeder and anestrous cows.
The serum calcium levels were significantly
decreased (P<0.05) in anestrous (7.31±0.02
mg/dl) cattle as compared to estrus. However,
these values were non-significantly different between repeat breeder and cattle
having estrus phase. The concentrations of serum phosphorus were significantly
higher (P<0.01) in normal estrual (4.99±0.08 mg/dl) as compared to repeat
breeder (3.90±0.06
mg/dl) and anestrous (3.82±0.04
mg/dl) Cholistani cattle. Mean serum MDA (nmol/ml) levels of repeat breeder
(2.68±0.18) and anestrous (2.54±0.22) were significantly (P<0.01) higher than the estrous (1.71±0.03) cattle.
Moreover, the serum nitric oxide levels (μmol/L) were also increased
significantly (P<0.01) in
repeat breeder (58.28±4.01) and anestrous (61.40±9.40) than the normal estrous
(31.67±6.71) cattle. The ratio of Ca: P in normal cyclic animals was
lower (1.73:1) as compared to the anestrous animals (1.92:1). It can be
concluded from the present study that the level of Ca: P should also be near to
1.5:1 for better reproductive performance.