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Responses of Blood Metabolites and Proteins to Different Vitamin A Levels in Korean Native Steers
 
Tao Wang1,6, Kyung-Hoon Lee2, U-Suk Jung2, Yong-Cheng Jin3, Sang-Bum Lee2, Jae-Sung Lee2, Jin-Hee Hwang2, Ji-Na Lim2, Min-Jeong Kim2, Seong-Ho Choi4, Man-Ho Choi5 and Hong-Gu Lee*,2
 
1College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, P.R.China; 2Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Seoul 143-701, Korea; 3Department of Animal Science, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, People Republic of China; 4Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Korea; 5Future Convergence Research Division, Korea Institute of Science and Technology, Seoul 136-791, Korea; 6Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, Jilin Agricultural University, Changchun, 130118, P. R. China
*Corresponding author: hglee66@konkuk.ac.kr
 

Abstract   

This study was conducted to determine the correlation between vitamin A restriction and serum metabolites as well as global proteins expression. Forty-eight animals were randomly divided into three groups and given different vitamin A supplementation: High (4.8 IU/g feed), Medium (2.43 IU/g feed) and Low (1.14 IU/g feed). Blood was collected individually via the external jugular veins. Results showed that the concentrations of Ca, total cholesterol, cortisol and cortisone, and the activity of 11beta-hydroxysteroid dehydrogenase (11-HSD) type 2 varied among the groups. An up-regulated (hibernation protein 25) and four down-regulated proteins (gelsolin a, alpha-1-microglobulin/bikunin precursor, transthyretin, and complement factor B) were identified in a proteomics study. These data demonstrated that vitamin A deficiency had considerable effects on the serum levels of several metabolites and global proteins expression and these metabolites and proteins may be used as physiological markers in the study of adipogenic differentiation prevention by vitamin A.

Key words: Korean native steers, Metabolomic, Proteomic, Vitamin A

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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