The Association of Single Nucleotide
Polymorphism in the
IGF1, IGF2 and IGF1R with Antler Yield in
Sika Deer
Feifei Yang, Pu Zhang, Lijun Huo, Hasan Riaz,
Liguo Yang, Shujun Zhang and Jiajun Xiong*
College of Animal
Science and Technology, Key Laboratory of Agricultural Animal Genetics,
Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan
430070, China *Corresponding author: xjiajunhau@yahoo.com.cn
Abstract
Insulin-like growth factors (IGFs)
are involved in a variety of physiological processes including cell
proliferation, differentiation, and apoptosis through paracrine and autocrine
mechanisms. In the present study, Insulin-like growth factor 1 (IGF1), Insulin-like growth factor 2 (IGF2) and Insulin-like growth factor 1 receptor (IGF1R)
has been implicated as candidate genes for the regulation of antler yield in
Sika deer population. Polymerase chain reaction-single strand conformation
polymorphism (PCR-SSCP) and DNA sequencing methods were employed in screening
for genetic variation among these candidate genes. We identified three novel
single nucleotide polymorphisms (A214G in intron 2, C246T in intron 4, and C65T
in exon 16) in IGF1R gene, without
harboring any SNP in other genes. The restriction site
Hhal was used for A214G, C246T and
C65T sites for further genotyping by Polymerase chain reaction-restriction
fragment length polymorphism (PCR-RFLP). Three genotypes were observed in each
of these sites in Wusan Sika deer (AG>AA>GG, CT>TT>CC, CT>CC>TT) and Dongfeng
Sika deer population (AG>GG>AA, CT>TT>CC, CC>CT>TT) respectively. There was a
trend towards improved antler yield in polymorphisms in IGFR2. Although there
was no significant difference (P>0.05) in these two Sika deer population this is
the first report revealing association between Insulin-like growth factors and
antler production in Sika deer population. This information would serve IGFs as
a genetic marker for the selection of higher antler producers in Sika deer
population.