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Saccharum officinarum Derived High Molecular Mass Glycoproteins as Native Biological Response Modifiers in Chickens
Mian Muhammad Awais1,2,*, Masood Akhtar1,3,*, Zafar Iqbal1 and Faqir Muhammad4
1Immunoparasitology Laboratory, Department of Parasitology, University of Agriculture, Faisalabad, Pakistan; 2Sub-campus Jhang, University of Veterinary and Animal Sciences, Lahore, Pakistan; 3Faculty of Veterinary Sciences, Baha-ud-Din Zakariya University, Multan, Pakistan; 4Department of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan
*Corresponding authors:;


Present study reports the biological response modifying (BRM) activities of sugar cane (Saccharum officinarum L.) derived high molecular mass glycoproteins (HMMGs) in chickens. HMMGs were recovered from sugar cane juice by size exclusion chromatography. The electrophoretic analysis of HMMGs sample revealed the presence of eight different glycoprotein fractions with molecular weights ranging from 82.81 to 255.56 kDa. These HMMGs were evaluated for their BRM activities in terms of cellular and humoral immune responses and production performance. Results showed that HMMGs significantly enhanced (P<0.05) the in vivo and in vitro lymphoproliferative responses to phytohaemagglutinin-P and Concanavalin-A, respectively in chickens. Significantly elevated B- cell mediated immune response in terms of antibody titres to sheep red blood cells, Newcastle disease and Infectious Bursal disease vaccines (P<0.05) in HMMGs administered chickens were also detected. Production performance was determined in terms of feed conversion ratios that were significantly improved (P<0.05) in chickens administered with HMMGs as compared to those of control group. On the whole, results suggested that HMMGs have immunostimulatory potential and can be used as native BRM agents in chickens to boost up their immunity and production performance.

Key words: Biological response modifiers, Chickens, Glycoproteins, Saccharum officinarum


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)