Biofilm Formation and Biofilm-Associated Genes
Assay of Staphylococcus aureus Isolated from Bovine Subclinical Mastitis
in China
Jian-zhong He1,2, An-qi Wang1,
Gang Liu1, Jian Gao1, Tariq Ali1
and Bo Han1,*
1College of Veterinary
Medicine, China Agricultural University, 100193 Beijing, China;
2Key Laboratory of Tarim Animal Husbandry Science &
Technology of Xinjiang Production & Construction Corps, 843300
Xinjiang Alar, China;
*Corresponding author:
hanbo@cau.edu.cn
Abstract
Staphylococcus aureus is one of the most common
pathogens responsible for contagious bovine mastitis. Genes involved in biofilm formation is a special
defensive mechanism of this pathogen to combat the host immune response and
remain stable in hostile environment. The present study was designed with objectives to investigate strategies
involving biofilm formation and biofilm associated genes (BAGs) of S. aureus
strains, and to assess the consistency of two phenotype test methods. One
hundred and two S. aureus strains were isolated from bovine subclinical
mastitis cases from 32 commercial dairy farms in nine provinces of China. These
isolates were screened for biofilm-producing capacity by Congo Red Agar (CRA)
and Semi Quantitative Adherence Assay (SQAA) methods. Thirteen BAGs including
rbf, SigB, SasG, icaA, sarA, icaR, icaD, clfA, clfB, fib, fnbpB,
bap and fnbpA were amplified
by PCR assay. The results of current study revealed that rbf (95.1%)
and SigB (94.1%) were the most
prevalent BAGs, followed by SasG (89.2%), icaA (88.2%),sarA (87.3%), icaR
(84.3%), icaD (82.5%), clfA (64.7%), clfB (45.1%), fib
(43.1%) and fnbpB (19.6%).However, bap and fnbpA
genes were not detected in any strain. By
CRA method, 78.4% strains of S. aureus produced biofilm and 48.0% of
strains were biofilm-positive by SQAA. Therefore, thedata
concluded that majority of S. aureus strains were capable to produce
biofilm, controlled by eleven associated genes, and CRA detection rate was
higher than SQAA for biofilm producing capacity of S. aureus.