The Polymorphism in the
IGF1R Gene is Associated with Body Weight and Average Daily Weight
Gain in
Pomeranian Coarsewool
Ewes
Witold Stanisław Proskura* and Małgorzata
Szewczuk
Laboratory of Molecular Cytogenetics, West
Pomeranian University of Technology, Szczecin, Poland *Corresponding author: witold.proskura@zut.edu.pl
Abstract
The aim of this study was to investigate the
association of five single nucleotide polymorphisms (SNPs) in
GHR (growth hormone receptor),
LEP (leptin),
IGF1 (insulin-like growth factor 1), and IGF1R
(insulin-like growth factor 1 receptor) genes with body weight at day 1, 33 and
90 of age and average weight gain at 1-33, 33-90 and 1-90 days of age in
Pomeranian Coarsewool sheep. Blood samples were collected from 100 ewes derived
from the only flock of this breed in Poland, and after DNA isolation, five
selected loci were genotyped with use of appropriately designed PCR-RFLP assays.
The g.122A>G in the GHR gene and the
g.251G>A in the LEP gene were
monomorphic, so only the effects of g.367G>T in the
LEP gene, g.271C>T in
IGF1 gene, and g.195C>T
in IGF1R gene were investigated. The statistical analysis showed no
association of the g.367G>A in LEP gene and g.271C >T in IGF1 gene
with the selected growth parameters. In contrast, the g.195C>T polymorphism in IGF1R gene was significantly associated
(P<0.001) with body weight and average daily weight gain. The TT genotype
was linked to the highest values, while the CC genotype was linked to the
lowest values, of the all analyzed traits. Thus, this study indicated the
g.195C>T SNP as a potential genetic marker for growth traits. Nevertheless that
effect should be investigated in other sheep breeds in order to confirm if that
SNP can be used for marker-assisted selection.