Gene Expressions in Major Organs between Enshi
Black and Landrace Pigs after
Streptococcus suis Infection
Uma Gaur1,2§, Hua-Yu Wu1,2§,
Zheng Feng1,2, Xue-Hai Yang1,2, Rui Guo1,2,
Jun-Jing Wu1,2, Kui Li3, Mei-Lin Jin4,
Shu-Qi Mei 1,2 and Gui-Sheng Liu1,2,*
1Institute
of Animal Science and Veterinary Medicine, Hubei Academy of
Agricultural Sciences, Yaoyuan No. 1, Nanhu, Hongshan District,
Wuhan 430064, China; 2Hubei Key Laboratory of Animal
Embryo Engineering and Molecular Breeding, Yaoyuan No. 1, Nanhu,
Hongshan District, Wuhan 430064, China; 3Beijing
Institute of Animal Science and Veterinary Medicine, Chinese Academy
of Agricultural Sciences, Malianwa, Haidian District, Beijing,
100193, China; 4College of Animal Science and Veterinary
Medicine, Huazhong Agricultural University, Shizi Mountain No. 1,
Nanhu, Hongshan District, Wuhan 430070, China *Corresponding author: guisheng_liu1964@yahoo.com
Abstract
Streptococcus suis 2 (SS2)
is an important zoonotic agent, and its pathogenesis has been extensively
studied recently. Our earlier study showed that there were different responses
between pig breeds after SS2
infection, which is similar to the previous observation in two mice lines. Bases
on our previous transcriptome sequencing profiles, 15 target genes in spleen,
lymph node, brain, lung, and liver were compared between Enshi black and
Landrace breeds after SS2 infection,
by qPCR in this study. Gene Resistin
was up-regulated in lymph and spleen of both breeds, whereas it was
significantly down-regulated in lung and liver of Enshi black.
IL-1b and
CD300c was significantly up-regulated
in the brain and lymph of Enshi black and the liver of Landrace, whereas
CXCL10 was significantly up-regulated
in the brain and liver of Enshi black, and the lymph of Landrace pig. Gene
NPG3 was highly induced in the brain
and lymph of Enshi black, but not so high in Landrace.
S100A8,
S100A9 and S100A12 were
significantly increased in the brain of Enshi black but S100A9 was significantly decreased in the lymph node of Enshi.
MMP9 in the brain was up-regulated in
Enshi black whereas it was down-regulated in Landrace. We observed differential
performances from 5 immunoglobulin genes, especially
IGLV5 and IGKV6. Our
results suggested that
Enshi black and Landrace have significant differences of
innate and adaptive immune responses after
SS2 infection. The results could lay
foundation for further deep study of resistance/ susceptibility mechanism in
pigs.
Key words:
Enshi black and Landrace pigs,
Gene expression, Immune response
Streptococcus suis