Association between Growth Hormone Single
Nucleotide polymorphism and Body Weight in Four
Saudi Camel (Camelus dromedarius) Breeds
Mohamed Afifi1,2*, Ehab MR Metwali1,3 and
Peter H Brooks4
1Department
of Biological Sciences, Faculty of Science, King Abdul Aziz
University, North Campus, PO Box 11508, Jeddah, 21463, Saudi Arabia;
2Department of Biochemistry, Faculty of Veterinary
Science, Zagazig University, Egypt; 3Genetic Branch,
Faculty of Agriculture, Suez Canal University, Ismailia 41522,
Egypt; 4School of Biological Sciences, Faculty of Science
and Environment, Plymouth University, Plymouth, PL4 8AA, UK;
*Corresponding author: mafifi@kau.edu.sa
Abstract
The aim of this study
was to evaluate the relationship between growth hormone gene (GH) polymorphism
and estimated body weight in Arabian camels. This was achieved by sequencing and alignment of the
growth hormone (GH) gene in four
Saudi Arabian camel breeds (Majaheem, Saheli, Waddah and Homor); searching for
single nucleotide polymorphisms (SNPs) and
correlating them with estimated body
weight. The polymerase chain reaction-restriction fragment length polymorphism
(PCR-RFLP) method was used to detect SNPs in the genotype of 200 animals from
each breed. Thirteen SNPs (two insertion and eleven substitution)
were detected in the Majahem breed, and
one was detected in the Waddah and Homor breeds each at position 419 (C419T).Two SNPs (C419T and T450C) were detected in the Saheli breed. Of these
the T450C SNP was associated with increased estimated body weight. Both male and
female Saheli camels with the CC genotype had higher body weights than the CT
and TT genotypes (P≤0.05).The SNP T450C, which was detected only in camels of
the Saheli breed, was correlated with greater body weight. Consequently, this
SNP may be a useful marker in the selection of camels for higher growth rate and
meat production.
Key words: Body weight, Growth hormone
gene polymorphism, Saudi camels