1Department
of Physiology and Pharmacology 2Department of Pathology,
University of Agriculture Faisalabad; 3The University of
Faisalabad, Faisalabad; 4Faculty of Pharmacy and
Alternative Medicine, Islamia University, Bahawalpur, Pakistan
Abstract
The present study was conducted to evaluate the
lipid lowering effect of a herbal mixture (containing garlic, lemon, ginger,
apple vinegar and honey) in hyperlipidemic adult male albino mice. Animals
were divided into six groups. Except normal control group, which was kept on
routine mice feed, the rest of the groups were provided with atherogenic diet
for 0-15 days (lead-in period) to induce hyperlipidemia. After that period
mixture was fed to hyperlipidemic albino
mice at the dose level of 1.5, 2.5 and 3.5 ml/kg BW to three treated
groups, respectively, for 15-60 days as cellulose replacement in atherogenic
diet. Simvastatin (synthetic lipid lowering drug, as reference standard) at the
dose rate of 0.6mg/kg BW was fed to the
hyperlipidemic albino mice of treated control group for 15-60 days as
cellulose replacement in atherogenic diet while untreated control group was kept
on atherogenic diet as such. Blood samples were taken and serum was tested for
lipid profile parameters at day 0, 15, 30, 45 and 60 after the initiation of
experiment. The results suggested that the values of percentage reduction
induced after administration of mixture, 3.5 ml/kg and simvastatin, 0.6 mg/kg,
respectively, at post treatment day 60, are non-significantly (P>0.05) different
(51.63 and 68.61 for TL, 49.61 and 60.26 for TGs, 59.54 and 64.72 for TC and
65.75 and 66.44 for LDL-c. Similarly, respective percentage increase, 20.38 and
26.25, for HDL-c were also mutually non-significant. Therefore, this is
concluded that mixture, 3.5 ml/kg and simvastatin, 0.6 mg/kg, are equieffective
in treating hyperlipidemia in male albino mice.
Key words:
Antihyperlipidemic activity,
High density lipoprotein,
Low density lipoprotein,
Mixture,
Simvastatin, Total cholesterol,
Total lipids, Triglycerides