Genetic Fingerprint of Unilocular Hydatidosis in Egyptian Camels and
Humans Using Nested PCR
Nashwa O Khalifa1,
Hanem F Khater2* and Marionette Z Nassief3
1Zoonoses
Department; 2 Parasitology Department, Faculty of
Veterinary Medicine, Benha University, Toukh, 13736, Egypt; 3Meat
Hygiene Department, Animal Health Research Center, Benha Branch,
Benha,13111, Egypt *Corresponding author: hafkhater@yahoo.com;
hanem.salem@fvtm.bu.edu.eg
Abstract
The pulmonary and hepatic hydatid cyst fluids
were collected from 540 slaughtered camels and 5 human cases in Qalyubia
Governorate, Egypt. The prevalence of infection of cystic echinococcosis among
camels was 120 (22.2%). The fertility rates of the isolated cysts form camels and humans were 64.5
and 100%, respectively.A nested
polymerase chain reaction was used for amplification of mitochondrial NADH 1
gene of Echinococcus granulosus complex in fertile cysts obtained from
camels and humans, respectively. Two pairs of primers (EGL1 and EGR2) and (EGL3
and EGR4) were used in 2 amplification steps. First, the outer pair of primer
originated from a highly conserve region of NADH1 gene generate a primary 435 bp
PCR product. Second, a pair of internal (nested) primer (EGL3 and EGR4),
designed to the annealing site of primers (EGL1 and EGR2) yield similar
diagnostic amplified DNA bands of molecular size marker at 276 bp in all
examined cysts obtained from camels and humansindicating a zoonotic relationship. This study confirms similar
fingerprinting patterns of Echinococcus granulosus complex in camels and
humans in Qalyubia Governorate, Egypt. Nested PCR for diagnosis of E.
granulosis had been used for the first time in Egypt, as far as we know.
Key words:
Camel,
DNA fingerprint, Echinococcus granulosus, Human, Nested PCR