An Anatolian Akbash shepherd dog died suddenly, without any clinical signs in Konya, Turkey. Performing necropsy, pathological examination, a culture test, laboratory tests and a multiplex-PCR of bacteria isolated from the dog revealed an anthrax and identified the bacteria as Bacillus anthracis (B. anthracis). The chromosomal gene sequence of bacteria was 99% identical in the GenBank under accession numbers CP002091 (B. anthracis str. H9401), CP001598 (B. anthracis str. A0248) and CP001215 (B. anthracis str. CDC684). Antimicrobial susceptibility test was performed and cefuroxime, cefquinome, sulfamethoxazole-trimethoprim, chloramphenicol, tetracycline and rifampicin resistance were seen in isolate. In this case, how the dog was infected with B. anthracis could not be determined.

Key words: Antimicrobial susceptibility, Bacillus anthracis, Dog, PCR, Sequence analysis