Ileal and Cecal Microbial Population and
Short-Chain Fatty Acid Profile in Broiler Chickens FedDiets
Supplemented with Lignocellulose
M Bogusławska-Tryk*, R
Szymeczko, A Piotrowska, K Burlikowska and K Śliżewska1
Department of Animal Physiology, Faculty of
Animal Breeding and Biology, University of Technology and Life
Sciences, Mazowiecka 28, 85-084 Bydgoszcz, Poland; 1Institute of Fermentation Technology and Microbiology, Lodz University
of Technology, Wolczanska 171/173, 90-924 Lodz, Poland *Corresponding author: monika.boguslawska-tryk@utp.edu.pl
Abstract
The study was conducted to evaluate the
performance indices, gut microbiota, short-chain fatty acid (SCFA) and lactic
acid concentration and pH value in ileal and cecal digesta of 42-day-old
broilers fed a diet supplemented with lignocellulose. A total of 48, 21-day-old
male Ross 308 chickens were divided into 4 treatment groups with 6 replicates
per treatment. Experimental diets were based on maize, wheat, triticale, soybean
meal and varied in the amount of lignocellulose: 0, 0.25, 0.5 and 1.0%. There
was no significant effect of lignocellulose inclusion into the diet on broiler
performance indices. Ileal Lactobacillusspp. as well as ileal and cecal
Bifidobacteriumspp. populations increased (P<0.05) and the number of
Escherichia coli and
Clostridiumspp. significantly decreased in birds fed diets supplemented with
lignocellulose. The sum of SCFAs and lactic acid in both intestine segments as
well as acetic and propionic acids in ileal and lactic acid level in cecal
digesta of chickens fed a diet with 0.5% lignocellulose were significantly
higher compared to the control birds. The results show that inclusion of
lignocellulose in the chicken diet, especially at a dose of 0.5%, promotes the
growth of Lactobacillusspp. and
Bifidobacterium spp., reduces the number of
E. coli and Clostridiumspp. as well as enhances the
concentration of SCFAs and lactic acid with no substantial effect on the pH of
ileal and cecal digesta. The intensity of fermentation and substantial amounts
of the lactic acid and individual SCFAs, particularly acetic and propionic
acids, may suggest the prebiotic effect of lignocellulose on the broiler
gastrointestinal tract.