Butachlor Induced Clinico-Hematological and Cellular
Changes in Fresh Water Fish Labeo rohita (Rohu)
Abdul Ghaffar1* Riaz Hussain2*,
Ahrar Khan3, Rao Zahid Abbas2 and Muhammad
Asad1
1Department
of Life Sciences (Zoology), The Islamia University of
Bahawalpur-63100; 2University College of Veterinary and
Animal Sciences, The Islamia University of Bahawalpur-63100,
Pakistan; 3Department
of Pathology, Faculty of Veterinary Science, University of
Agriculture, Faisalabad-38040, Pakistan *Corresponding authors:zoologistag@yahoo.com;driazhussain@yahoo.com
Abstract
The present study was conducted to explore the
clinico-hematological and genotoxic effects in fresh water fish (Labeo rohita)
exposed to various
concentrations of butachlor (chloroacetanilide
herbicide). For this purpose 30 fish weighing about 500-600 g were
randomly allocated to five equal groups (A-E) and treated with various
concentrations (0,
0.25, 0.50, 0.75
and 1.00 mg/L) of butachlor. Blood samples were collected from each fish
after 48, 72 and 96 h of post treatment and fresh thin blood smears were made
for cellular abnormalities. All the fish kept in group A did not reveal any
clinical and behavioral ailments. However, various clinical signs such as rapid
breathing, gasping at the water surface, restlessness, opercular movement, loss
of equilibrium and erratic swimming were observed at higher concentrations (0.75
and 1.00 mg/L) of herbicide. The results showed that the erythrocyte,
hemoglobin, hematocrit and lymphocyte values were significantly (P<0.05)
decreased while significantly increased total leukocyte counts were recorded.
Significantly increased incidence of different morphological and nuclear
abnormalities such as pear shape erythrocyte, microcyte, tear shape erythrocyte,
erythrocytes with micronuclei, lobed, blebbed and notched nuclei and cells with
nuclear remnants were observed. The
results suggested that butachlor poses serious clinico-hematological and
mutagenic effects in fish and may be hazardous to the other species when
those are exposed to this herbicide.