Evaluation of Extracts of Seeds of
Syzygium cumini L. for
Hepatoprotective Activity Using CCl4-Induced Stressed
Rats
M Islam, K Hussain*, A
Latif, FK Hashmi, H Saeed, NI Bukhari, SS Hassan, MZ Danish and B
Ahmad
University College of
Pharmacy, University of the Punjab, Allama Iqbal Campus,
Lahore-54000, Pakistan *Corresponding author:hussain_761@yahoo.com
Abstract
The hepatoprotective effects of seeds of
Syzygium cumini L. (Family:
Myrtaceae), presumed to be effective
in treating gastrointestinal diseases of animals, have not been investigated
before, particularly in liver damage caused by infections, chemicals and
xenobiotics. Therefore, this aimed at investigating the hepatoprotective effects
of methanol extracts of plant seeds in chemically (CCl4) induced
stress rats. Adult male, Sprague Dawley
rats (n=30) were randomly segregated into 5 equal groups i.e., group-I
(control), group-II (silymarin treated; 1.0 mg/kg BW), group-III (extract of
Syzygium cumini seeds treated; 250
mg/kg BW), group-IV (extract treated; 500 mg/kg) and group-V (CCl4
treated; 1.5 mg/kg). Rats were treated with respective treatments for 14
consecutive days. At day 14, four hours after the last dose, an oral dose of CCl4
(1.5 mg/Kg, 1:1 in olive oil) was administered to all the groups, except animals
in the control group. Subsequently, 24h later, blood samples and liver tissues
were collected for biochemical analysis and histopathology, respectively. The
values of liver function markers were found to be significantly (P<0.05) lower
while serum protein level was significantly higher in control and treated groups
as compared to that of the CCl4 treated group. Histological
examination of liver tissues also indicated that the extract of
Syzygium cumini seeds in both the
doses, and silymarin protected the liver from CCl4-induced stress. It
was concluded that extract of seed of Syzygium cumini has hepatoprotective activity.