This study was designed to evaluate antihistaminic and anticholinergic activities of Murraya koenigii leaves. To observe the activities ethanolic (Mk.Eth) and aqueous (Mk.Aq) extracts of M. koenigii through in vitro analysis, isolated guinea pig ileum, rabbit trachea and jejunum tissues were used and tissues responses were recorded by Power Lab data acquisition system connected with isotonic or isometric transducers. Mk.Eth and Mk.Aq showed rightward shift in histamine concentration response curves at 0.1-0.3mg/ml, when tested on isolated guinea pig ileums. Both extracts also relaxed carbachol (1µM) induced pre contracted isolated rabbit trachea and jejunum tissue, dose dependently but a more potent effect was observed with aqueous extract of M. koenigii. Keeping in view all the results, presence of antihistaminic and anticholinergic activities in aqueous and ethanolic extracts of M. koenigii leaves can be strongly suggested that provides sound basis for application of M. koenigii in airways and gastrointestinal disorders.

Key words: Anticholinergic, Antihistaminic, Asthma, Diarrhea, Murraya koenigii