Single Nucleotide Polymorphisms in Lactoferrin
Gene are Associated with Lactoferrin Content in Milk and Somatic
Cell Count in Deoni (Bos
indicus) Cows
Arun Pratap Singh1*, KP Ramesha1,
S Isloor2, P Divya1, Akhila Rao1, M
Basavaraju1, DN Das1 and U Munde1
1Genetics
Laboratory, Dairy Production Section, SRS of NDRI, Adugodi,
Bangalore-560030, India; 2Department
of Veterinary Microbiology, Veterinary College, Hebbal,
Bangalore-560024, India *Corresponding author: drapsndri@gmail.com
Abstract
Lactoferrin, a
glycoprotein, is an important antimicrobial molecule present in
milk which plays a vital role in antimicrobial defence of the udder in bovines.Inthe
present
investigation, polymerase chain reaction- single-strand conformation
polymorphism (PCR-SSCP) studies
werecarried
outin
122 Deoni(Bos
indicus) cows to detect SNPs oflactoferrin gene and to analyze the
association between the observed polymorphisms with milk lactoferrin
content and Somatic Cell Count (SCC).
The PCR-SSCPanalysis revealed a total of 20 different
variants in the entire coding regions
of the lactoferrin gene. Analysisofexons2, 6 and 8 of lactoferrin gene
revealed4, 3 and 5 unique SSCPpatterns,
respectively.
The PCR-SSCPanalysisofexons3,9, 14and15revealed
two unique SSCPpatterns.
Comparison of nucleotide sequences of lactoferrin gene of the Deoni cows
with taurine cattle revealed a total of 39 point mutations, 25 of which were
found to be in coding region. Conceptualized translation of nucleotide sequence
revealed 15 amino acid changes. The SSCP variants of exons 2 and 6 had significant (P<0.05) effect on milk
SCC. The SSCP variants of exon 3 and exon 15 showed significant effect on
lactoferrin content. The SCC and lactoferrin content in Deoni cows
was highest in 4th and above parity group. Somatic Cell Count
was lowest in early stage of lactation. Parity was found to have highly
significant (P<0.01) effect on both milk SCC and lactoferrin
content. The observed association between
SSCP variants in lactoferrin gene
with milk SCC, and milk lactoferrin content suggests the possibility of using
these genetic variants in lactoferrin gene as prognostic markers for
selection of animals for high lactoferrin content, low SCC and mastitis
resistance.