Aim of the present study was to know the current prevalence of brucellosis at different Government and private Livestock Farms in Punjab, Pakistan. For this purpose, 2275 serum samples were collected and subjected to various diagnostic tests including Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT), i-ELISA and c-ELISA. Data thus collected was interpreted and subjected to Binary Logistic regression analysis to know the difference among different sources. Sensitivity and specificity values of these different tests were also determined. Seroprevalence of bovine brucellosis was significantly (P<0.05) higher in private livestock farms as compared to government farms through all the diagnostic tests applied. Sensitivity of SAT was higher in cattle (73.46%) as compared to the other species. c-ELISA was slightly more sensitive than i-ELISA. However, the specificity of all the tests in all species was 100%.

Key words: Brucellosis, Epidemiology, Livestock, Sensitivity, Specificity