The present study was conducted to determine stability of Bovine viral diarrhea virus (BVDV) RNA stored at different temperatures. A total of 6 blood samples obtained from a private cattle farm, which were found to be antigen positive (Ag+) by direct ELISA method, were used in this study. BVDV Ag+ samples were stored separately at 4, 21 and 37^oC for 1 month. The samples were analyzed on the 0, 1^st, 2^nd, 3^rd and 4^th weeks by ELISA for the presence of BVDV Ag and by RT-PCR for the presence of BVDV RNA. Stability of BVDV RNA was calculated using maximum concentration (C_max) and area under the curve (AUC) as kinetic parameters of each sample. All of the samples were found positive both by ELISA and RT-PCR on each week. C_max values of BVDV RNA for the storage temperatures of 4, 21 and 37°C were 356, 346 and 338 ng/µL respectively, and AUC_0→4 values for the same temperatures were 1151, 1106 and 1077 week.ng/µL respectively. It was determined that storage at different temperatures for one month does not statistically influence the kinetic parameters of BVDV RNA (P>0.05). In conclusion, it can be expressed that storage of BVDV RNA at 4, 21 and 37°C for one month has no effect on the stability of BVDV RNA.

Key words: AUC, BVDV, C_max, ELISA, RT-PCR