Antimicrobial Activity of 4 Novel Cyclic Peptides
against a Panel of Reference and Multi-Drug Resistant Clinical
Strains of Animal Origin
CS Cabassi1*, S Taddei1, S
Cavirani1, A Sala1, D Santospirito1,
MC Baroni2 and AA Romani3
1Dipartimento di Scienze
Medico-Veterinarie, Università
degli
Studi di
Parma,
via del Taglio 10, 43126 Parma, Italy; 2Dipartimento
di Medicina Clinica e Sperimentale, Università
degli
Studi di
Parma,
via Gramsci 14, 43126 Parma,Italy;
3Dipartimento Emergenza-Urgenza e Area Medica Generale e
Specialistica, Azienda Ospedaliero-Universitaria di
Parma,
via Gramsci 14, 43126 Parma,Italy *Corresponding author: clotildesilvia.cabassi@unipr.it
Abstract
In this study,
antimicrobial activity of novel cyclic peptides coded as P1, P3, P5 and P8
against reference bacterial and fungal strains and clinical multidrug resistant
(MDR) bacterial strains of animal origin was
investigated. Good antimicrobial
activity against Pseudomonas aeruginosa, Escherichia coli, Malassetia
pachydermatis and Candida albicans was found, while against
Gram-positive bacteria, the minimum
bactericidal concentration of peptide that killed >90% of bacteria (MBC90)
was >100μg/mL. Excellent activity was noticed for P3
against E. coli MDR clinical strains (MBC90 1.6-12.5μg/mL),
and against P. aeruginosa MDR clinical strains (MBC90
3.2-12.5μg/mL). The peptides readily permeabilized P. aeruginosa
membranes as evaluated by propidium iodide dead-stain assay. The peptides showed
salt dependence with hemolytic activity close to 30% at 100μg/mL. The results so far obtained indicate good
and rapid antimicrobial activity of three peptides P1, P3 and P8 that prompt for
further improvement in light of a potential topical therapeutic use.