Cloprostenol and PMSG Administration Promote
Estrus Synchronization,
Uterine Development
and FSHR Expression in Mice
Wei Suocheng1,3, Gong Zhuandi2,
Chen Shien3*, An Lifeng2, Zhang Taijun3,
Luo Yongfu3 and Dai Haiou3
1The
key bio-engineering and technology laboratory of National
Nationality Commission;
2Medicine College; 3Life Science and Engineering
College, Northwest University for Nationalities, Lanzhou, China *Corresponding author: chensn_huining@163.com
Abstract
The present study aimed to investigate the
effects ofPMSG
and cloprostenol (CLO) on
the estrus synchronization, uterine development as
well as follicle stimulating hormone receptor (FSHR) expression in
animals. 105
female KM mice, 30 days old and body weight of 25.28±2.26g, were assigned into seven subgroups (n=15). Mice in PMSG-1 (pregnant
mare serum gonadotrophin), PMSG-2 and PMSG-3 subgroups were injected with 10, 20 and 40IU PMSG. Mice in CLO-1, CLO-2 and
CLO-3 subgroups were intramuscularly injected with 10, 15 and 20μg
cloprostenol. Western-blotting
was implemented to detect expression of uterine FSHR proteins. The results
showed that 93.33% and 66.67% of synchronized mice of CLO and PMSG groups
displayed estrus in 18.68-37.59h, respectively. Estrus numbers, estrus onset time (EOT) and estrus rates in CLO and PMSG groups were
greater than in control group (CG) (P<0.05).EOT in CLO and PMSG groups were 19.88±2.91
hours and 34.84±5.05 hours.Uterine weights of PMSG group
were higher than that of CLO and CG groups. Uterine wall
thickness (UWT) of PMSG group was higher than that of CLO and CG groups.
Uterine horn longitudial
diameter (ULD) in
experimental mice was greater than CG. ULD in PMSG-2 and PMSG-3 were
significantly greater than CG. Uterine horn transverse
diameter (UTD) of CLO-1, PMSG-1,
PMSG-2 and PMSG-3 subgroups were significantly larger than CG (P<0.05). Expression levels of FSHR proteins were
different among all subgroups. FSHR protein levels in CLO and PMSG increased
lightly in comparison with CG. In conclusion, PMSG
and CLO treatments could synchronize estrus of mice, enhance the uterine
development of mice.
Efficacy of CLO on estrus synchronization was greater than
PMSG. The effects of PMSG
on uterine development were stronger than cloprostenol. This is benefit for modulating the reproductive
functions of animals.
Key words:
Cloprostenol,
Estrus synchronization,
Follicle stimulating Hormone receptor, Mice, Pregnant
mare serum Gonadotropin, Uterine development