EpidemiologicalStudy of Diarrheagenic Escherichia coli
Virulence Genes in Newborn Calves
Emad A Hashish1*, Hend M El Damaty2,
Yasmine H Tartor3 and Ahmed M Abdelaal4
1Department
of Clinical Pathology; 2Department of Animal Medicine,
Infectious Diseases; 3Department of Bacteriology,
Mycology and Immunology; 4Department of Animal Medicine,
Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt; *Corresponding author: eagad@vet.zu.edu.eg
Abstract
Diarrheagenic Escherichia coli (DEC) are
considered one of the major etiological agents of neonatal calf diarrhea (NCD).
One hundred calves (2-21 days-old) suffering from diarrhea in Sharkia Governorate,
Egypt were used in this study. Six E. coli virulence genes were
characterized by multiplex PCR including; heat-stable enterotoxins (STa; 33.3%),heat-labile
enterotoxin (LT; 30%),
two Shiga toxins: (Stx1; 86.67%),
(Stx2; 26.67%)
produced from Shiga toxin producing E. coli (STEC)and two
enterotoxigenic E. coli (ETEC) adhesions (F5; 13.3%) and
(F41; 16.67%).Each isolate was found
to carry one or more virulence genes. Clinicobiochemical examination showed
significant (P<0.01) increase in serum total protein, globulin, alanine
aminotransferase (ALT), aspartate aminotransferase (AST), creatinine, blood urea
nitrogen (BUN), and potassium levels. Meanwhile; serum albumin, A/G ratio,
sodium and chloride levels were significantly (P<0.01) decreased. Multiplex PCR
was a useful quick diagnostic tool for characterization and identification of
E. coli virulence genes. Shiga toxin
genes increase the risk for zoonotic STEC infection. Mixed E. coli
infection is strongly associated with
the reported cases of diarrhea due to the presence of several virulence
genes in some isolates.
Key words:
Calves,
Clincobiochemical assays,
Diarrheagenic E. coli, Multiplex PCR, Virulence genes