Hematopoietic Potential of Polysaccharides
Isolated from Angelica
sinensis against ACE Inhibitor Induced Anemia in Albino Rats
Syeda Kiran Zahra, Bilal Aslam*, Ijaz Javed, Tanweer Khaliq,
Junaid Ali Khan and Ahmad Raza
Institute of Pharmacy, Physiology and
Pharmacology,
University of Agriculture, Faisalabad-38040, Pakistan *Corresponding author:cba933@gmail.com
Abstract
Angiotensin converting enzyme (ACE) inhibitors
are widely used in treatment of cardiovascular diseases. Their long term use
causes development of anemia in patients with these diseases. Present study was
designed to evaluate the protective hematopoietic potential of
Angelica sinensis polysaccharides
(ASP) against ACE inhibitor-induced anemia along with their toxic effects on
kidney and liver cells. Adult male albino rats were randomly divided into five
equal groups (n=6). Group I was control group and group II was administered with
ACE inhibitor (20 mg/kg/day) to induce anemia. In group III, ACE inhibitor was
administered (20 mg/kg/day) in combination with erythropoietin (EPO, 100
IU/kg/each). Group IV was administered with ASP at the dose rate of 1 g/kg/day.
In Group V, ACE inhibitor (20 mg/kg/day) was administered in combination with
ASP (1 g/kg/day). After 28 days, blood and tissue samples were collected for
hematological and histopathological analysis respectively. The results showed
that ACE inhibitors significantly reduced (P<0.05) the hemoglobin value (Hb),
packed cell volume (PCV), red blood cell (RBC) count, mean corpuscular volume
(MCV) and mean corpuscular hemoglobin (MCH) values. In group treated
with ASP, there was significant (P<0.05) increase in Hb value and RBC count. The
combination of ACE inhibitor and ASP led to the significant (P<0.05) reduction
in Hb, PCV, RBC count, MCV and MCH values. While histopathological examination
of liver and kidney cells showed a mild degree of toxicity in ASP treated group.