Relationship between
Pro-Inflammatory Cytokines, IL-10 Anti-Inflammatory Cytokine and
Serum Proteins in Healthy Lambs and with Diarrhea
A
Fernández1*, D Marteles1, M Ruiz de Arcaute1,2,
D Lacasta1, T Conde1,3 and A Loste1
1Departamento de Patología Animal, Facultad de
Veterinaria, Universidad de Zaragoza, Miguel Servet 177,
50013-Zaragoza, Spain; 2Gabinete Técnico Veterinario,
Isla Conejera, bajos, 50014-Zaragoza, Spain; 3Veterinarios
Especialistas en Equidos, Zaragoza-Spain *Corresponding author:
afmedica@unizar.es
Abstract
We determined the
levels of the cytokines IL-6, IL-1β, IFN-γ and IL-10 in fifteen lambs during the
first month of life. The correlation between these cytokines and serum protein
fractions was studied. In addition, the concentration of these cytokines in
fourteen lambs with diarrhea was determined. IFN-γ (73.85±121 pg/ml) peaked at
day 1, IL-6 (7910±2329 pg/ml) and IL-1β (5234±2461 pg/ml) peaked at day 4, and
IL-10 (63.4±5.2 pg/ml), peaked at day 28. At day 11 the highest correlation
between IL-6 and IL-1β was identified. An increase (P<0.01) in IL-1β
concentration was detected in diarrheic lambs. There was a positive correlation
between IL-6, IL-1β and total protein levels in sick lambs and also between IL-6
and total globulins and IL-1β and total globulins. These results indicate that
with the exception of IL-10, cytokine concentrations in healthy lambs follow
time-dependent evolution. The higher concentration of IL-1β detected in
diarrheic lambs could be used as are reliable inflammatory marker in ovine
pathology. Further work is required to understand the role of cytokines in both
physiological and pathological processes.