Pathobiological
Findings of Epitheliocystis in Giant Grouper (Epinephelus lanceolatus) in Taiwan
HK Chang1,
KP Li2, YL Tsai1, YC Wu1, JH Shien2,
SL Hsuan1 and CC Lin1*
1Graduate Institute of
Veterinary Pathobiology; 2Department of Veterinary
Medicine , National Chung Hsing University, 250
Kuo Kuang Road, Taichung, 402, Taiwan *Corresponding author:
vpcclin@dragon.nchu.edu.tw
Abstract
A Giant groupers (Epinephelus lanceolatus)
farm in southern Taiwan displayedsymptoms of hypoxia with mortality around 3.5%. Wet mount examination
revealed mottled gills with abundant oval-shaped cysts as the main
characteristic lesions. Sporadic occurrence of diffusely distributed white nodules in the
kidney was also observed. Through histopathological examination, basophilic, cytoplasmic cysts were found
diffusely scattered in the secondary lamellae. The contents of
the cysts produced a dark magenta hue under Macchiavello stain, indicating the
presence of chlamydia-like organisms. Focal to multifocal granulomatous,
necrotic lesions were observed in the kidney and spleen, and were the result of
Vibrio spp.and Photobacterium spp.
infection. DNA samples were extracted
from the gills and the resulting product was a 298 bp fragment with a nucleotide
sequence that was 98% homologous with Simkaniaceae spp.
As the result, the final diagnosis was
epitheliocystis with secondary bacterial infection.