Over-Expression of Rab1 Gene during Infectious
Bursal Disease Virus Infection in Layer Chicken
Jinyou Ma1,2, Yan Yu1,2,
Huihui Zhang1, Haizhen Mo1, Changbo Ou1,*,
Xuannian Wang3 and Xingyou Liu1,3
1College
of Animal Science; Henan Institute of Science and Technology,
Xinxiang 453003, Henan, China; 2Institute of Animal
Virus; Henan Institute of Science and Technology, Xinxiang 453003,
Henan, China; 3Department of Life Sciences and
Technology, XinXiang University, Xinxiang 453003, Henan, China *Corresponding author: ouchangbo2004@163.com
Abstract
Infectious bursal disease virus (IBDV) can cause
immuno-suppression and morbidity in chickens and mainly replicate in the bursa
of Fabricius, spleen, thymus and other lymphoid tissues. Rab1 gene is one of Rab
GTPases and mainly deal with intracellular protein transport between endoplasmic
reticulum (ER) and Golgi. In the present study, SPF layer chickens were
artificially challenged with IBDV and the relationship between Rab1 protein gene
expression and virus replication was explored by real-time PCR. The aim of the
current study was to initially understand dynamic expression of Rab1 during the
IBDV infection and offer basic data for further study of IBDV pathogenesis. The
data showed that the content of Rab1 peaked on day 3 P.I. in infected group and
the Rab1 gene levels were about 2.7 times those of the mocked-infected group.
Likewise, the contents of VP2 of chickens in the infected group peaked on day 4
post-infection. After that, the VP2 gene levels slowly dropped until chickens
were sacrificed. Moreover, there were two amino acid mutation sites were found
on sites 61 and 159 in the infected groups and Lysine (K) and threonine
(T) were mutated into glutamate (E) and alanine (A), respectively. The change of
mRNA expression level of Rab1 gene from chick bursa post IBDV infection
suggested that Rab1 might play a vital role during IBDV replication.