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Optimization of Quality Control Factors for Indigenous Mycoplasma Gallisepticum Bacterin Preparation and Their Impact on Immunoprophylaxis in Broilers
Asim Raza1, Arfan Ahmad1, Masood Rabbani1, Altaf Mahmood2, Muhammad Younus3, Zakir Ali2 and Abdul Ahad4,*
1Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan; 2Livestock and Dairy Development Department, Govt. of Punjab, Pakistan; 3Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan; 4Department of Microbiology and Veterinary Public Health, Chittagong Veterinary and Animal Sciences University Chittagong, Khulshi–4225, Bangladesh


To inactivate locally isolated Mycoplasma gallisepticum (MG), phenol, formaldehyde and binary ethylenimine were optimized for their concentrations and exposure time. Their effect on immunoprophylaxis induction was then assessed by using different levels of immunogen/PCV and different adjuvants. 0.10M, 0.125% and 0.6% concentrations of binary ethylenimine, formaldehyde and phenol inactivated MG colonies within 24, 8 and 4 hours respectively. Formaldehyde inactivated vaccine with 1.5% Immunogen/PCV and montanide oil as adjuvant induced significantly (P<0.05) higher anti MG antibody titer than other locally prepared vaccines. Conclusion is that montanide oil adjuvated indigenous formalized bacterin can induce desired immune response.

Key words: Adjuvants, Bacterins, Broilers, Inactivants, Mycoplasma gallisepticum (MG)


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)