PAKISTAN VETERINARY JOURNAL

Pak Vet J, 2016, 36(2): 227-229

Optimization of Quality Control Factors for Indigenous Mycoplasma Gallisepticum Bacterin Preparation and Their Impact on Immunoprophylaxis in Broilers

Asim Raza¹, Arfan Ahmad¹, Masood Rabbani¹, Altaf Mahmood², Muhammad Younus³, Zakir Ali² and Abdul Ahad⁴,*

¹Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan; ²Livestock and Dairy Development Department, Govt. of Punjab, Pakistan; ³Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan; ⁴Department of Microbiology and Veterinary Public Health, Chittagong Veterinary and Animal Sciences University Chittagong, Khulshi–4225, Bangladesh
*Corresponding author:ahadvet1969@yahoo.co.uk

Abstract

To inactivate locally isolated Mycoplasma gallisepticum (MG), phenol, formaldehyde and binary ethylenimine were optimized for their concentrations and exposure time. Their effect on immunoprophylaxis induction was then assessed by using different levels of immunogen/PCV and different adjuvants. 0.10M, 0.125% and 0.6% concentrations of binary ethylenimine, formaldehyde and phenol inactivated MG colonies within 24, 8 and 4 hours respectively. Formaldehyde inactivated vaccine with 1.5% Immunogen/PCV and montanide oil as adjuvant induced significantly (P<0.05) higher anti MG antibody titer than other locally prepared vaccines. Conclusion is that montanide oil adjuvated indigenous formalized bacterin can induce desired immune response.

Key words: Adjuvants, Bacterins, Broilers, Inactivants, Mycoplasma gallisepticum (MG)

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)