Gambogic Acid Inhibits Hsp90 Expressions in
Thiram-Induced Tibial Dyschondroplasia
Fazul Nabi1,2,§, Kun Li1,§,
Muhammad Shahzad1,3, Zhaoqing Han1, Ding Zhang1,
Jingying Liu1 and Jiakui Li1*
1College
of Veterinary Medicine, Huazhong Agricultural University, Wuhan
430070, PR China; 2Faculty of Veterinary & Animal
Science, Lasbela University of Agriculture Water and Marine Science
Uthal Balochistan 90150, Pakistan; 3Department
of Pathobiology, College of Veterinary & Animal Sciences, The
Islamia University of Bahawalpur, Pakistan;
*Corresponding author: lijk210@sina.com
Abstract
Tibial Dyschondroplasia (TD) is an important leg
problem in fast growing birds that disturbs the proximal tibial growth plate. A
study was conducted to evaluate the effects of gambogic acid (GA) on hsp-90
expressions and antioxidant capability in thiram-induced TD chicken. One hundred
and fifty day-old commercial broiler chicks were distributed into three groups:
Control (A), thiram-induced (B) and GA treated (C). Tibial bone samples were
collected at day 7 & 14 to evaluate hsp-90 expressions and liver samples were
procured at the end of experiment to determine the antioxidant enzymes. As
compared to control, the results demonstrated that significant increased hsp-90
gene expressions (P<0.05) in thiram-induced TD chicks on day 7 & 14 contributing
to the progression of TD while a decrease in antioxidant capacity of liver on
day 14. On administering the GA, the hsp-90 expressions were down-regulated and
restored the antioxidant capacity of liver significantly (P<0.05). In
conclusion, the GA ameliorated the growth plate vascularization in TD-afflicted
chicks which provides a new clinical usage of this promising agent against TD.