Lipopolysaccharide (LPS)
Induces Matrix Metalloproteinase-2 and -9 (MMP-2 and MMP-9) in
Bovine Dermal Fibroblasts
Ahmet Akkoc1,*, M. Mufit Kahraman1,
Alper Vatansever1, Elcin Gunaydın2 and Ezgi
Akdesir1
1Uludag
University, Faculty of Veterinary Medicine, Department of Pathology,
16059, Gorukle, Bursa, Turkey; 2Veterinary Control
Central Research Institute, Breeding Disease Diagnosis Laboratory,
06020, Etlik, Ankara, Turkey;
*Corresponding author:
aakkoc@uludag.edu.tr
Abstract
Bovine digital dermatitis (BDD) is one of the
most important problems in dairy herds. The exact etiology is unknown but it is
believed that bacteria have cardinal roles. Matrix metalloproteinases (MMPs) are
a group of enzymes responsible for the degradation of extracellular matrix
proteins. The plausible role of these enzymes in the formation of ulcerative
lesions in various tissues in humans is widely known. Ulcers on the plantar skin
of hind feet are the prominent lesions in early stage of BDD. The main objective
of this study was to determine whether MMP-2 and -9 enzymes are enhanced
following LPS challenge in bovine dermal fibroblasts. Skin samples were obtained
from two Holstein-Friesian cows after slaughtering. Primary bovine dermal
fibroblasts were isolated from foot skin and then exposed to 5 µg/ml LPS.As result, we found that LPS stimulates the release of MMP-2 and MMP-9
enzymes in bovine dermal fibroblasts. When compared with controls, immunoblots
revealed significant increase in MMP-2 (by 56%, P<0.005) and MMP-9 (by 74%,
P<0.005) enzymes.Zymographic analyses
demonstrated LPS challenge resulted with the release of higher amount of active
MMP-2 and –9 enzymes by 37% and 48%, respectively. These data indicate LPS may
be an important factor in the pathogenesis of BDD by causing local MMP-2 and
MMP-9 enzyme release.
Key words:
Bovine dermal fibroblast,
Digital dermatitis,
Immunoblotting,
Lipopolysaccaride, Matrix metalloproteinase