Phenotypic and Genotypic Characterization of Staphylococcus
aureus Strains Associated with Bovine Mastitis and Nasal
Carriage of Workers in Contact to Animals in Algeria
1Ecole Nationale Supérieure Vétérinaire, 16000,Alger, Algérie; 2Institut des Sciences
Vétérinaires, Université Blida1, 09000, Blida, Algérie; 3Département
de Biologie Cellulaire et Moléculaire, USTHB, 16000, Alger, Algérie;
4Service de Microbiologie, CHU Mustapha-Bacha, 16000,
Algérie; 5Inserm U851, IFR 128, CNR des Staphylocoques,
Université Lyon 1, 69008, Lyon, France; 6Centre de
biologie Est, Hospices Civils de Lyon, 69500 Bron, France; 7Laboratoire
des Biotechnologies de la Reproduction, Institut Vétérinaire Blida,
09000, Algérie;
*Corresponding author: akkou.m@gmail.com
Abstract
Staphylococcus aureus is a major bovine mastitis pathogen responsible for
heavy economic losses in dairy industry. Identification of epidemiological
aspects associated with bovine mastitis may be helpful in treatment and
management decisions. Due to high concern of zoonotic infections, we describe
here, the distribution and antimicrobial susceptibility patterns of S. aureus
from both cows with mastitis and nasal carriage of workers in contact to
animals. Up to 38% workers were nasal carriers. Besides, S. aureus was
isolated among 74% dairy herds suffering from mastitis, within 29.8% of mastitic
quarter-milk samples. The isolates were tested for antimicrobial resistance,
gyr, mecA, mecC and agr alleles. The gene gyr
was detected in all S. aureus strains, 91 (77.7%) belonged to agr
specificity group I, 11.9% affiliated to group II, 10 (8.5%) were agrIII,
and 1.7% human derived isolates to the group IV. agr I was dominant in
both human and animal isolates with 60 and 91%, respectively. Four human
isolates harbored mecA gene, while no mecA or mecC genes
were found in bovine derived isolates. Overall, 92% human isolates and 86.5% of
cows’ derived strains were resistant to penicillin G. The resistance against non
beta-lactam antibiotics was considerably greater in human than cows’ derived
isolates, while different patterns of resistance share the same ecological
niches. The high association of penicillin resistance to S. aureus in
bovine mastitis highlights periodic surveillance of antibiotic resistance in
livestock.
Key words:
agr, Drug resistance,
Livestock workers,
Mastitis, S. aureus