Genetic Properties of the New Pseudorabies Virus
Isolates in China in 2012
H Hu, T Yu, J Fan1, S Cheng, C Liu, F
Deng, N Guo, B Wu and QG He*
State Key Laboratory of Agricultural
Microbiology, Division of Animal Infectious Diseases, College of
Veterinary Medicine, Huazhong Agricultural University, 430070 Wuhan,
China;1Wuhan
Keqian Animal Biological Products Co., Ltd., Wuhan, China *Corresponding author: he628@mail.hzau.edu.cn
Abstract
Since the beginning of 2012, a disease, that
emerging with the clinical characteristics of neurologic symptoms and high
mortalities in piglets, was observed in many farms in China. Clinical samples of
brains and lungs, that collected from sick piglets, abortion and death fetuses
in 8 different farms, were examined. The virulent and immunogenicity related
genes of pseudorabies viruses (PRV) were detected from these samples and five
PRV strains were isolated successfully. The amino acid sequence analysis of the
gE, gB, gC, TK RR1 and RR2 genes showed that, these strains could form an
independent cluster together despite of some amino acid changes, with the
homology being 99.4~100%, 99.3~100%, 99.9~100%, 98.4~100%, 98.9~99.7%,
98.7~100%, respectively. If compared with the relative genes of PRV Bartha and
Ea strain, these isolates have some unique amino acid insertions, deletions and
changes which do not exist among Chinese strains isolated previously. These
findings indicated that these newly isolates did not derive from the current
vaccine strains. Oppositely, they might evolve from field strains and the
process was still obscure. These results might partially explain the
prophylactic failure and emergency of pseudorabies in some areas of China.
Key words:
Glycoprotein B,
Glycoprotein C,
Glycoprotein E,
Phylogenetic analysis, Pseudorabies virus