Dynamic Investigation of
Porcine Epidemic Diarrhea and Analysis Sequence of Spike Gene in
South China over the Past Five Years
Zhili Li1, Zhaozhou Li1, Xuan Li1,
Li Cao1, Hongli Gao1, Yanshan Chen2, Xiduo Zeng2, Baoli Sun3,
Qingmei Xie3, Yingzuo Bi3 and Jing-Yun Ma3,*
1College of food and bioengineering, Henan
University of Science and Technology, 263, Kaiyuan Road,
Luoyang 471023, Henan, People’s Republic of China; 2Guangdong
Wen’s Foodstuff Group Co., Ltd., Yanjiang Street, Xinxing 527400,
Guangdong, People’s Republic of China; 3College of Animal Science, South China Agricultural
University, Tianhe District, Wushan Road, Guangzhou 510642,
Guangdong, People’s Republic of China
*Corresponding author:
mjy000713@gmail.com
Abstract
The study executed a large-scale molecular epidemiological investigation of
porcine epidemic diarrhea (PED) and five-yearlong monitoring of nine swine herds
with the outbreak of diarrhea in south China. Porcine epidemic diarrhea virus
(PEDV) exists all year round with varying degrees of mortality to suckling
piglets, the highest mortality was in 2011 and followed by 2012, the lowest
mortality was in 2013, with the mortality showed an increasing trend year by
year during 2014 to 2015. It was noteworthy that the pregnant sows assumed the
highest morbidity, followed by nursing sow while the nursed piglets and the
growing pigs had lower morbidity. The current epidemic strains in south China
were divided into two groups, Group 1 and Group 2 by generating the phylogenetic
tree of thirty-five spike (S) genes during 2011-2015. Group 1 had 6.92-7.21%
mutation rate while Group 2 owned 4.18% mutation rate when compared with CV777.
The strains in Group 2 felled into the same branch with the previous Chinese
isolates from 2004 while the strains in Group 1 had a close relationship with
the United States strain. Interestingly, clinical morbidity caused by two types
of PEDV strains were quite different, strains in G2-2 caused relatively minor
mild clinical manifestations, presenting a smaller mortality and shorter onset
period, while strains in G1-2 and G1-3 is caused by an outbreak of acute
infectious diseases, showed a larger mortality and longer period especially PEDV
strains in G1-3. It was also indicated that the effect way to prevent PEDV
should focus on reduce the morbidity of pregnant sows.