Serotypes, Genotypes, Virulence Factors and
Antimicrobial Resistance Genes of Escherichia coli
Isolated in Bovine Clinical Mastitis from
Eastern China
Javed Memon1,
Jam Kashif2, Nazir Hussain2, Muhammad Yaqoob2,
Asghar Ali2, Rehana Buriro2, Jamila Soomro2,
Mohammad Farooque Hassan3, Benazir Sahito2 and
Fan Hongjie1*
1College
of Veterinary Medicine, Nanjing Agricultural University, Nanjing
210095, P.R. China; 2Sindh Agricultural University,
Tandojam, Pakistan; 3Shaheed Benazir Bhutto University of
Veterinary and Animal Sciences, Sakrand, Sindh, Pakistan *Corresponding author: fhj@njau.edu.cn
Abstract
Coliform mastitis is still significantly problematic disease to treat and
control in practice, which is testament to the intricacy and mutability of the
condition.
We examined 103 mastitis
E. coli isolates collected from 22 dairy farms in eastern China, for
their serotypes and prevalence of virulence and antimicrobial resistance genes.
Of the sixteen serotypes characterized through serum agglutination test, O39
followed by O92 and O123 were the most predominant serovars. The genotyping of the isolates was also determined by
using ERIC-PCR. Phylogenetic analysis of DNA fingerprints was performed by using
SPSS data editor, which yielded ten distinct genotypes (A-J). 14 virulence genes and 10 antimicrobial resistance
genes were checked in all 103 isolates by PCR assay. Most prevalent virulence
genes were, TraT, FimH, papC, iucD, F4
(K88) and sfa; but
F17A, F41, stx1, intimin, CNF1, CNF2, LT
and ST were not present in any
isolate. Among all investigated resistance genes, 48% isolates carried
CTX-M and
qnrS. In addition, tetA,
tetB,
sul1, sul2 were also found
in high frequency. Statistical analysis revealed an unconditional association
between virulence and resistance genes as the P<0.05. To our knowledge, this is
most updated report on serotypes, genotypes, prevalence of resistance and
virulence genes, and their significant association with each other in mastitis
E. coli isolates from eastern China.
Key words:
E. coli, Genotypes,
Mastitis,
Resistance genes,
Serotypes, Virulence