Genotypic Characterization of
Bordetella bronchiseptica Strains Isolated from Stray and Pet Dogs
Zafer Sayin1*, Asli Sakmanoglu1,
Osman Erganis1, Uckun Sait Ucan1, Hasan
Huseyin Hadimli1, Zeki Aras2, Gokcenur
Sanioglu2 and Alp Aslan Coskun3
1Department
of Microbiology, Faculty of Veterinary Medicine, Selcuk University,
42075, Selcuklu, Konya, Turkey; 2Department of
Microbiology, Faculty of Veterinary Medicine, Aksaray University,
68100, Aksaray, Turkey; 3Department of Internal Medicine,
Faculty of Veterinary Medicine, Cumhuriyet University, 58140, Sivas,
Turkey;
*Corresponding author:zafersayin@gmail.com
Abstract
Bordetella bronchiseptica (B. bronchiseptica)
is the most important pathogen associated with
kennel cough in dogs. The presence of
B. bronchiseptica in pet dogs and
shelter dogs with clinical respiratory disease was investigated in present study. The genetic relatedness among the strainswas determined to
evaluate the role of stray dogs in spread of
B. bronchiseptica to pet dogs by detection of virulence genes such
as filamentous hemagglutinin (fha), pertactin (prn) and
dermonecrotic toxin (dnt). We also performed the
random amplified polymorphic DNA (RAPD) assay. A
total of 96 B. bronchiseptica were isolated from
stray and pet dogs. The
fha, prn anddnt virulence genes were detected in 86, 83.3 and 61.4%
strains, respectively by polymerase chain reaction (PCR)
techniques. The most common genotype from stray and pet dogs
was fha+prn+dnt+ as detected in
37.5% and 11.4% of all the strains, respectively.The RAPD assay showed that 3 different patterns
were obtained from 96 B. bronchiseptica
strains. Sixty one (63.5%) of them were clustered in one main group and then
further placed in another 2 sub-groups by RAPD assay.
Genetic association was seen between the
B. bronchiseptica strains from stray and pet dogs. In conclusion, this study
revealed thatB.
bronchiseptica is present at a higher rate in
stray dogs than pet dogs. Stray dogs might have a significant role in the
transmission of B. bronchiseptica to pet dogs.