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Effects of Selenium-Enriched Probiotics on Heart Lesions by Influencing the mRNA Expressions of Selenoproteins and Heat Shock Proteins in Heat Stressed Broiler Chickens
Alam Zeb Khan1,2, Shahnawaz Kumbhar1, Muhammad Hamid1, Samreen Afzal3, Fahmida Parveen1, Yunhuan Liu1, Hao Shu1, Berhe Mekonnen Mengistu1 and Kehe Huang1*
1Institute of Nutritional and Metabolic Disorders of Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing 210095, China; 2Gomal College of Veterinary Sciences, Gomal University, Dera Ismail Khan 29050, Pakistan; 3College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
*Corresponding author:


Selenium is one of the most vital trace elements regulating various body functions. Herein, we observe the effects of selenium-enriched probiotics on heart lesion in broiler chickens under high ambient temperature and explore the underlying mechanisms. Four different groups of broiler chickens were fed a corn-soybean basal diet having no Se supplementation (Con group), basal diet with the addition of probiotics (P group), a basal diet with Se supplementation in the form of sodium selenite (SS group, 0.30mg Se/kg) and basal diet with the addition of selenium enriched probiotics (SP group, 0.30mg Se/kg). The results showed that P, SS, or SP supplementation significantly (P<0.05) up-regulated mRNA expression of selenoproteins (GPx1, GPx4) and down-regulated heat shock proteins (Hsp60, Hsp70 and Hsp90) in heart as compared to Con, P and SS diets. Herein, we suggest that SP product can serve as a feasible nutritive supplement, capable of protecting the heart from toxic effect of oxidative stress in summer season.

Key words: Broiler chickens, Heart lesions, Heat shock proteins, Heat stress, Selenium-enriched probiotics, Selenoproteins


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)