Antimicrobial Resistance of
Escherichia coli Isolated from Tibetan Piglets Suffering from White
Score Diarrhea
Hui Zhang1§, Mujeeb Ur Rehman1§,
Kun Li1, Houqiang Luo1,3, Yanfang Lan1,
Fazul Nabi1, Muhammad Shahzad4, Shucheng Huang1,
Xinyu Liu1, Khalid Mehmood1,4, Muhammad Kashif Iqbal1 and Jiakui Li1,2*
1College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China;
2Laboratory of Detection and Monitoring of Highland Animal Disease,
Tibet Agriculture and Animal Husbandry College, Linzhi 860000 Tibet, People's Republic of China; 3Animal Science Department, Wenzhou Vocational College of
Science & Technology 325006, People's Republic of China;
4College of Veterinary & Animal Sciences, The Islamia
University of Bahawalpur 63100, Pakistan *Corresponding author: lijk210@sina.com
Abstract
The aim of this study was to investigate the drug
resistance profile of Escherichia coli(E.
coli) isolated from Tibetan piglets having white score
diarrhea in Qinghai Tibetan Plateau of
China. A total of 81 E. coli
strains were isolated from 83 fecal samples. While commonly used antimicrobial
drugs and polymerase chain reaction (PCR) were used to detect the drug resistant
and representative drug resistance genes. The
results showed that the isolated E. coli
was highly resistant to medemycin (100%), aztreonam (87.65%), tobramycin
(55.56%), and kanamycin (46.91%), and lower resistant to cefoxitin (40.74%) and
ceftazidime (30.86%). Isolates tolerant to 3 or more than 3 antimicrobial drugs accounted
for 87.65% and were classified as multidrug resistant (MDR). The
prevalent genes detected were blaTEM
and aac-Im, belonging to
beta-Lactamases and aminoglycosides,
classrespectively. In conclusion,
the antimicrobial resistance of E. coli
from free-ranging Tibetan piglets showed a high tolerant rate than other regions
of China. The results of the present study revealed that, the use of single
antibiotic for a long period of time was the main reason that increased the
antibiotic resistance in Tibetan pigs. Therefore, the insensitive antimicrobial
drugs should be avoided in clinical treatment of bacterial diseases.