An Enzyme-Linked Immunosorbent Assay for Brucella
Specific Antibody and Real-Time PCR for Detecting
Brucella Spp. in Milk and Cheese in Şanlıurfa, Turkey
Serap Kiliç Altun1, Akın Yiğin2,
Sevil Erdenliğ Gürbilek3, Semra Gürbüz4*,Mehmet Demirci5, Oktay Keskin3 and Osman
Yaşar Tel3
1Department
of Food Hygiene and Technology, 2Department of Genetic,
3Department of Microbiology, Faculty of Veterinary
Medicine, University of Harran, Şanlıurfa – Turkey; 4Higher
School of Tourism and Hotel Management,University of
Mardin Artuklu, Gastronomy and Culinary Art, Mardin– Turkey; 5Cerrahpaşa
Medical Faculty, Medical Microbiology, University of Istanbul,
Istanbul–Turkey *Corresponding author: semragurbuz@gmail.com
Abstract
The objective of this study was to investigate the presence of anti-Brucella
antibody and Brucella spp. DNA in cow, sheep and goat milk and in Urfa cheese
collected from markets and bazaars in Şanlıurfa, located in southeast of Turkey.
A total of 258 samples consisting of 178 raw milk (48 cow milk, 65 sheep milk
and 65 goat milk) samples and 80 Urfa cheese samples were investigated. Anti-Brucella
antibody was detected by indirect ELISA (i-ELISA), and the presence of Brucella
spp. DNA was screened by real time Polymerase Chain Reaction (RT-PCR). 16.6% of
the cow, 6.1% of the goat and 6.1% of the sheep milk and 16.25% of the cheese
samples were found as positive for brucella antibodies by i-ELISA. The RT-PCR
assay amplified Brucella DNA from 18.75, 7.6 and 6.1% cow, goat and sheep milk
samples respectively. Brucella DNA was amplified from 22.5% cheese samples. The
11.2% and 13.9% of the samples were found as positive by i-ELISA and RT-PCR
respectively. This study indicates that milk and milk products consumed in
Şanlıurfa poses a risk to public health in terms of brucellosis. The combining
usage of both i-ELISA and RT-PCR methods could lead to more reliable results to
detect anti-Brucella antibody and Brucella spp. DNA from milk and cheese
samples.