Identification of Coagulase Gene in
Staphylococcus aureus
Isolates Recovered from Subclinical Mastitis in Camels
Amjad Islam Aqib1, Muhammad Ijaz1,
Riaz Hussain2*, Aneela Zameer Durrani1, Aftab
Ahmad Anjum3, Arif Rizwan1, Saba Sana3,
Shahid Hussain Farooqi1 and Kashif Hussain1
1Department
of Clinical Medicine, Faculty of Veterinary Sciences, University of
Veterinary and Animal Sciences, Lahore; 2Department of
Pathobiology, University College of Veterinary and Animal Sciences,
Islamia University, Bahawalpur-63100; 3Department of
Microbiology, Faculty of Veterinary Sciences, University of
Veterinary and Animal Sciences, Lahore, Pakistan
Abstract
The current study was conducted to estimate
coagulase positive Staphylococcus aureus
(S. aureus) being a nuisance
pathogen among mastitis etiology from subclinical cases of camel mastitis and to
investigate the association of assumed risk factors. A total of 235 subclinical positive milk samples were
collected from camels kept at different localities of Cholistan desert, Punjab
Pakistan by using Surf Field Mastitis Test (SFMT). Data regarding some possible
risk factors associated with subclinical mastitis were also collected and
analysed by chi-square techniques at 95% confidence interval. The study revealed
50.54% (235/465) prevalence of subclinical
mastitis in camels. Staphylococci were
cultured in 74.04% (174/235) of milk samples from subclinical camel mastitis.
Results showed 88.5% (154/174) prevalence of coagulase positive
S. aureus using slide and tube
agglutination techniques, while 54.02% (94/174) prevalence of coagulase positive
S. aureus was confirmed by PCR
technique.The quarter based prevalence of subclinical
mastitis was examined in 30.93% (520/1681) quarters, whereas 9.6% (179/1860)
quarters were blocked in camels.
Chi-square and Odd’s ratio analysis showed significant (P<0.05) association of
mastitis with age, parity and udder pathology while non significant association
with unhygienic condition of udder, lack of teat dipping practice, lactation
status and feeding management. It was concluded that
coagulase positive S. aureus was the major cause of mastitis in camel and
there was positive association of some risk factors with occurring of mastitis.