Induction of Immunosuppression in Broiler Chicken
upon Co-infection of Avian Adenovirus-4 with Low Pathogenic Avian
Influenza H9N2
Farooq Rashid1,2*, Muhammad Athar
Abbas1,2, Naila Siddique1,2, Saba Rafique1,
Samina Yasmeen1, Farida Mehmood1, Abdullah Shah2,
Muhammad Suleman3, Sohaib Roomi4 and Khalid
Naeem1
1National
Reference Laboratory for Poultry Disease; 2PARC Institute
of Advanced Studies in Agriculture, National Agricultural Research
Centre, Islamabad, Pakistan; 3Centre of Biotechnology and
Microbiology, University of Swat, Pakistan; 4Plant Tissue
Culture Lab, Biosciences Department, Comsats University, Islamabad,
Pakistan
Abstract
Despite the infectious nature of Angara Disease (AD), the disease has also been
clinically observed in association with other infectious diseases of poultry,
which alone may not be capable to cause high mortality. The present study was designed to evaluate the
pathology of co-infection of Avian Adenovirus-4 (AAV-4) and Avian Influenza
virus (AIV) serotype H9N2 on the health of broiler chicken. In this regard,
12-day old chicks were first experimentally infected with AAV-4 followed by
re-infection with AIV H9N2. The chickens infected only with AAV-4 showed virus
detection from primary and secondary lymphoid organs up to 7 days
post-infection. However, the duration of detection was extended up to 28 days
post AIV-H9N2 infection when these birds were co-infected with Avian Influenza
serotype H9N2. Moreover, a reduced serological response was observed against
AIV-H9N2 in the presence of AAV-4 infection in chicken in comparison with the
serological response against AIV H9N2 from healthy birds.