With the purpose of researching on the effect of Compound Kuqin total polysaccharides on T cell subsets and cytokines of blood in canines with canine parvovirus, the model was established, and 120 canines were separated into 6 groups, such as blank control group (Group Ⅰ), model group (Group Ⅱ), positive medicine group (Group Ⅲ), compound Kuqin total polysaccharides high dose group (Group Ⅳ), middle dose group (Group Ⅴ), low dose group (Group Ⅵ). We set the time point when the virus was injected into canines for 6 hours as day 0, and collected the blood sampling from the canine saphenous vein at different time. Finally, we detected the percentages of T lymphocyte subsets and the content of IL-2, IL-4 and IFN-. Compared with Group Ⅰ, the percentage of CD3+CD4+ T cells in Group Ⅱ decreased in 0-35d, CD3+ T cells decreased and CD3+CD8+ T cells increased in 21-35d, the content of IL-4 and IFN-γ decreased from day 7 to 21, IL-2 decreased from day 21 to 35; Compared with Group Ⅱ, the percentages of CD3+, CD3+CD4+ T cells in Group Ⅲ and Group Ⅳ increased in 7-28d, CD3+CD8+ T cells in Group Ⅳ and Ⅴ decreased in 7-35d, the content of IL-2 in the Group Ⅳ to Ⅵ increased in 14-28d, IL-4 and IFN-γ in the Group Ⅳ to Ⅵ increased in 0-35d. The result showed that the high dose Compound Kuqin total polysaccharides can enhance the secretion of IL-2, IL-4, IFN- and increase the percentages of CD3+, CD3+CD4+ T cells, and decrease the percentage of CD3+CD8+ T cells. Based on the results, we concluded that the total polysaccharides of compound Kuqin can regulate canine immune functions and improve the antiviral ability in parvovirus infection.

Key words: Canine parvovirus, Compound Kuqin, Cytokines, Polysaccharides, T cell subsets