Maggot Antimicrobial Peptide
Effect on TGF-β4 and TNF-α mRNA Expression in
Small Intestinal Mucosa
from Salmonellapullorum-Infected
Chickens
Wang ZT§1, Wang JG§2, Zhang
YS1, Zhang XL1, Xi JF1, Li CC1, Huang CF1and Shen H1*
1College
of Animal Science and Technology, Shihezi University, Xinjiang,
832003, PR China 2College of Agronomy, Shihezi
University, Xinjiang, 832003, PR China *Corresponding author: shenhong98@163.com
Abstract
To explore the effects of maggot antimicrobial peptide treatment of Salmonella
pullorum-infected chickens on the mRNA expression levels of TGF-β4 and TNF-α in
small intestinal mucosa, the duodenum, jejunum and ileum mucosa were isolated 3,
5 and 7 days after induced AMP, non-induced AMP, antibiotics or maggot treatment
and the TGF-β4 and TNF-α mRNA expression was evaluated by quantitative real-time
PCR. We found that mRNA of TGF-β4 was highly expressed in the duodenum, jejunum,
and ileum when Salmonella pullorum-infected chickens were treated with
antibiotics, non-induced AMP, and AMP, respectively (P<0.001). Similarly, TNF-α
mRNA was highly expressed in the duodenum and jejunum when Salmonella pullorum-infected
chickens were treated with induced AMP and maggots, respectively (P<0.001), and
the expression of TNF-α mRNA was higher in response to the use of induced AMP
than antibiotics on day 5 after treatment (P<0.05). Therefore, maggot AMP
functional efficiency, along with evaluation of TGF-β4 and TNF-α produced by
small intestinal mucosa, may be useful for the prevention and treatment of
Salmonella pullorum when developing new chicken feed additives.