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Maggot Antimicrobial Peptide Effect on TGF-β4 and TNF-α mRNA Expression in Small Intestinal Mucosa from Salmonella pullorum-Infected Chickens
 
Wang ZT§1, Wang JG§2, Zhang YS1, Zhang XL1, Xi JF1, Li CC1, Huang CF1 and Shen H1*
 
1College of Animal Science and Technology, Shihezi University, Xinjiang, 832003, PR China
2College of Agronomy, Shihezi University, Xinjiang, 832003, PR China
*Corresponding author: shenhong98@163.com
 

Abstract   

To explore the effects of maggot antimicrobial peptide treatment of Salmonella pullorum-infected chickens on the mRNA expression levels of TGF-β4 and TNF-α in small intestinal mucosa, the duodenum, jejunum and ileum mucosa were isolated 3, 5 and 7 days after induced AMP, non-induced AMP, antibiotics or maggot treatment and the TGF-β4 and TNF-α mRNA expression was evaluated by quantitative real-time PCR. We found that mRNA of TGF-β4 was highly expressed in the duodenum, jejunum, and ileum when Salmonella pullorum-infected chickens were treated with antibiotics, non-induced AMP, and AMP, respectively (P<0.001). Similarly, TNF-α mRNA was highly expressed in the duodenum and jejunum when Salmonella pullorum-infected chickens were treated with induced AMP and maggots, respectively (P<0.001), and the expression of TNF-α mRNA was higher in response to the use of induced AMP than antibiotics on day 5 after treatment (P<0.05). Therefore, maggot AMP functional efficiency, along with evaluation of TGF-β4 and TNF-α produced by small intestinal mucosa, may be useful for the prevention and treatment of Salmonella pullorum when developing new chicken feed additives.

Key words: Chickens, Intestinal mucosa, Maggots, Salmonella, TGF-β4, TNF-α

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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