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Pathological Investigations of Organ Affinity of Brucella Species and their Cross Species Transmission
R Akhtar1*, MN Anwar1, I Khan2, H El-Adawy3, A Aslam1, G Mustafa4, SF Rehmani4, MZ Saleem1 and S Naz5
1Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan; 2Section of Epidemiology and Public Health, College of Veterinary and Animal Sciences, Jhang, Pakistan; 3Friedrich Loeffler Institute, Jena, Germany; 4Quality Operational Lab, University of Veterinary and Animal Sciences, Lahore, Pakistan; 5Veterinary Research Institute, Lahore; *Corresponding author:


The present study was conducted to determine the localization of Brucella species within different aborted tissues. Another objective was to determine its cross species transmission of Brucella in three main districts of Punjab, Pakistan with significant bovine population. The results revealed the presence of Brucella abortus (23.3%) only and none of bovine fetus was positive for Brucella melitensis (0%) while lung was the most prevalent targeted tissue for Brucella as compare to liver and stomach respectively in both cattle and buffaloes fetuses. The results also indicated increased Brucella incidence in cattle fetuses (30 & 13.3%) than buffalo fetuses (16.6 & 6.66%) (P=0.034) by both AMOS PCR and IHC respectively. We also found significantly more positive cases by AMOS PCR than IHC (P=0.024). These novel findings about best specimen selection for isolation of Brucella and absence of Brucella melitensis in bovines of Punjab Pakistan can be helpful in designing brucellosis control programs.

Key words: Aborted fetuses, Bovine brucellosis, Liver, Lungs, Stomach


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)