Pathological Investigations of Organ Affinity ofBrucella Species and their Cross
Species Transmission
R Akhtar1*,MN Anwar1,
I Khan2, H El-Adawy3, A Aslam1,G Mustafa4, SF Rehmani4, MZ Saleem1
and S Naz5
1Department
of Pathology, University of Veterinary and Animal Sciences,
Lahore, Pakistan; 2Section of Epidemiology and Public
Health, College of Veterinary and Animal Sciences, Jhang, Pakistan;
3Friedrich Loeffler Institute, Jena, Germany; 4Quality
Operational Lab, University of Veterinary and Animal Sciences,
Lahore, Pakistan; 5Veterinary Research Institute, Lahore;
*Corresponding author:
raheela.akhtar@uvas.edu.pk
Abstract
The present study was conducted to determine the
localization of Brucella species
within different aborted tissues. Another objective was to determine its cross
species transmission of Brucella in
three main districts of Punjab, Pakistan with significant bovine population. The
results revealed the presence of Brucella
abortus (23.3%) only and none of bovine fetus was positive for
Brucella melitensis (0%) while lung
was the most prevalent targeted tissue for
Brucella as compare to liver and stomach respectively in both cattle and
buffaloes fetuses. The results also indicated increased
Brucella incidence in cattle fetuses (30 & 13.3%) than buffalo
fetuses (16.6 & 6.66%) (P=0.034) by both AMOS PCR and IHC respectively. We also
found significantly more positive cases by AMOS PCR than IHC (P=0.024). These
novel findings about best specimen selection for isolation of
Brucella and absence of
Brucella melitensis in bovines of
Punjab Pakistan can be helpful in designing brucellosis control programs.