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Coherence and Colonization Characteristics of Recombinant Lactobacillus under Simulated Gastric Conditions within Chicken GI tract and its impact on Chicken Growth
Syed Mohsin Bukhari1*, Maqsood Iram1, Tang Lijie1, Ma Sunting1, Han Le Mang1, Ghulam Abbas2, Irfan Baboo3 and Yijing Li1
1Department of Preventive Veterinary Medicine, Faculty of Veterinary Medicine, Northeast Agricultural University, Harbin, China; 2Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Northeast Agricultural University, Harbin, China; 3Department of Zoolog, Faculty of Basic Science, Government College University, Lahore, Pakistan
*Corresponding author:


The gastrointestinal tract (GIT) of chickens is massively occupied by pathogens and lactobacillus where lactobacillus may basically be used as symbiotic member. Particular species of lactobacillus such as Lactobacillus casei (Lc393) and Lactobacillus saerimneri (M11) regulating the function of immune system, as a probiotics and therapeutic agent produce protein against pathogens. In this study, we constructed a series of recombinant lactobacillus ppG-T7g10-ppT-ompC1-fimA1/Lc393 (POF1/Lc393), ppG-T7g10-ppT-ompC1-fimA1/M11 (POF1/M11), ppG-T7g10-ppT-ompC78-fimA78/Lc393 (POF78/Lc393) and ppG-T7g10-ppT-omp C78-fimA78/M11 (POF78/M11). For Examination, 70 day one old (SPF) chicks were divided into 5 batches (1Lc,78Lc,1M11,78M11 and PBS control) comprising 14 chicks in each batch. At day one, all batches were drenched orally with recombinants of 1.5x109 CFU/ ml, 1.5x1010 CFU/ ml, 1.5x1011 CFU/ ml and also drenched control with100ul of PBS respectively. After vaccination, all batches were challenged orally with 0.5ml of E.coli. The colonization and adherence ability were then examined in ileum, cecum and colon of chickens at different intervals. Results of recombinant lactobacillus POF1/Lc393 and POF78/Lc393 demonstrated that 1.5x109ml, 1.5x1010 ml and 1.5x1011 ml concentration of recombinants drenched respectively had no mortality while POF1/M11, POF78/M11 showed 20% mortality in all batches. Vaccine treated groups POF1/Lc393 and POF78/Lc393 showed significantly higher chicken growth performance (P<0.05) as compared with POF1/M11, POF78/M11 and control (PBS). The results determined that possibility to construction and colonization of these recombinants to be used as therapeutic agent and growth performance modulator in chickens.

Key words: Chickens, Colonization, Gastrointestinal tract, Probiotics, Recombinant lactobacillus


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)