The aim of the present study was to evaluate the pattern of antioxidants in broiler chicken that were vaccinated and experimentally challenged with Newcastle disease (ND) virus. A total of 300 healthy day-old Cobb broiler chicks were divided randomly into four equal groups. The chicks in group 1 and 2 were vaccinated with live B1-ND vaccine. Those in group 2 were injected with a killed vaccine and group 3 chicks received only the adjuvant of killed vaccine. The birds in groups 1, 2 and 3 were challenged with a velogenic NDV and those in group 4 were kept as control. Sampling was done on days 1,2,3,7 after vaccination and on 1, 2, 3,7,14, 21 post challenge. The parameters measured included TAC, MDA, vitamins (A, E, C) and trace elements (Se, Cu, Zn). There was oxidative stress in all groups except control. Significant decrease in TAC was confirmed in all groups except control. The most oxidative stress was clarified in group 1 and the rate of decline for TAC was more significant. There were not specific differences in serum MDA levels between four groups but totally they were respectively higher in the 1-3 groups. According to the results, no significant changes in serum levels of trace elements and vitamins were observed. Although there were significant differences of these trace elements and vitamins between groups on some days, no specific trends were observed. In conclusion the changes indicate that maximum production of oxidative radicals occurs when live vaccine and virus are used concurrently that could be because of more possible inflammatory reaction compared with the other groups.