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Evaluation of Antiviral Activity of Azadirachta indica (Neem) Bark Extract against Newcastle Disease Virus
M. Shahid Mahmood1, H Waqas Amir1, Rao Zahid Abbas2, Bilal Aslam3 and Azhar Rafique4*
1Institute of Microbiology; 2Department of Parasitology, University of Agriculture Faisalabad, Pakistan
3Department of Microbiology; 4Department of Zoology, GC University Faisalabad Pakistan
*Corresponding author:


In the present research, the in-vitro and in-ovo antiviral activity of Neem (Azadirachta indica) bark extract against Newcastle disease virus (NDV) was evaluated. Various dilutions of Azadirachta indica extract were used against NDV. In-vitro evaluation was done by performing spot assay and micro-hemagglutination test, while in-ovo antiviral activity was assessed by injecting the extracts in 11 days old embryonated eggs. During in-vitro evaluation, it was found that the stock solution and 1:2 dilution of Neem bark extract exhibit antiviral activity but at the same time these concentrations showed cytotoxic activity as well, while higher dilution (1:8) showed non-significant antiviral activity. Same was true for in-ovo evaluation, higher dilutions showed non-significant activity. Taking together, it may be concluded there was no significant difference in the antiviral effects of different concentrations of Neem bark extracts but the exposure time was a significant variable for cytotoxicity.

To Cite This Article: Mahmood MS, Amir HW, Abbas RZ, Rafique A and Aslam B, 2018. Evaluation of antiviral activity of Azadirachta indica (Neem) bark extract against Newcastle disease virus. Pak Vet J, 38(1): 25-28.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)